ADNI-2 Active Study Page

Welcome ADNI-2 Study staff, coordinators, and PI’s.

This section encompasses study specific tools for your reference. If you have any questions, comments, or new ideas please contact NCRAD by email or phone (800)526-2839.

General questions about the study, not related to the specimens should be directed to the ADNI website.

ADNI-2 Biological Sample Form

The biological sample form is maintained in the worksheet packet on the ADNI-2 web portal.

We respectfully ask that all completed forms be emailed (alzstudy@iu.edu) or faxed (317-278-1100) prior to shipment. We also ask that all shipments include a hard copy of each sample form.

Buffy Coat Instructions

After the lavender-top EDTA tubes have been centrifuged, the blood will be separated into layers. The buffy coat is the thin layer (.5 - .75mL) between the plasma and red blood cells. There should always be a buffy coat and it is usually white in color.

The buffy coat should be extracted from the lavender-top EDTA tubes and extraction should occur after the plasma has been transferred. Using sterile gloves and a sterile pipette, extract the buffy coat from one of the lavender-top EDTA tubes by going in a circular motion around the inside of the tube where the buffy coat is and aliquot it into one of the 2mL cryogenic vials. Repeat this process for the second lavender-top EDTA tube and the remaining, empty 2mL cryogenic vial. It is very normal to get some of the red blood cells when extracting the buffy coat and if red blood cells are not included then you have not gone far enough down the tube. Since some red blood cells will be included, then you will likely get 1 ml or more volume transferred to the cryovial. Be sure to complete all fields on the labels and secure each label to a cryogenic vial without obscuring any information.

Study Related Video Tutorials